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cd25 cd49d regulatory 208 t cell isolation kit  (Miltenyi Biotec)
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Miltenyi Biotec cd25 cd49d regulatory 208 t cell isolation kit
Cd25 Cd49d Regulatory 208 T Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti human cd49d antibody  (Miltenyi Biotec)
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Miltenyi Biotec anti human cd49d antibody
qPCR shows cholesterol biosynthesis is significantly increased in plasma cultured cells. ( A ) Main steps in cholesterol and fatty acid biosynthesis. Cholesterol biosynthesis begins with acetyl-CoA and proceeds via HMG-CoA reductase (HMGCR), forming mevalonate and downstream intermediates (mevalonate-PP, isopentenyl-PP, and squalene). Squalene epoxidase (SQLE) converts squalene to 2,3(S)-oxidosqualene, leading to lanosterol and cholesterol synthesis. Fatty acid biosynthesis also originates from acetyl-CoA, with acetyl-CoA carboxylase (ACC) producing malonyl-CoA, followed by fatty acid synthase (FASN) generating palmitate and complex fatty acids. SREBP1/2 transcription factors regulate these pathways, with SREBP1 driving fatty acid synthesis and SREBP2 controlling cholesterol biosynthesis. The LDL receptor (LDLR) mediates cholesterol uptake, maintaining lipid homeostasis. Made using Biorender. ( B ) mRNA levels of HMGCR (HMG-CoA reductase), SQLE (Squalene Epoxidase), LDLR (Low density lipoprotein receptor), SREBP1 and SREBP2 (Sterol regulatory element-binding protein 1 or 2) relative to GAPDH on day 8 of differentiation after CD34 + isolation. A parametric Brown-Forsythe and Welch test was performed to test for differences between groups. p < 0.05 was considered statistically significant ( n = 3, N = 3). ( C ) Example waterfall plots of cells the same donor on day 8 of erythroid differentiation, labelled against Band3 and <t>CD49d,</t> grown in medium containing AB serum, Plasma, or Plasma supplemented with CRL. Corresponding percentage of each stage (I-Proerythoblast, II-Basophilic, III-Polychromatic, IV-Orthochromatic, and V-Reticulocyte) is indicated next to the corresponding gate. D Shows percentage of erythroid cell types present on day 8 for each condition studied, quantified as displayed in panel above ( n = 3). Error bars represent the standard deviation.
Anti Human Cd49d Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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172yb cx3cr1 2a9 1 fluidigm 3172017b 173yb granzyme gb11 fluidigm 3173006b b 174yb cd49d 9f10 fluidigm 3174018b  (fluidigm)
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fluidigm 172yb cx3cr1 2a9 1 fluidigm 3172017b 173yb granzyme gb11 fluidigm 3173006b b 174yb cd49d 9f10 fluidigm 3174018b
qPCR shows cholesterol biosynthesis is significantly increased in plasma cultured cells. ( A ) Main steps in cholesterol and fatty acid biosynthesis. Cholesterol biosynthesis begins with acetyl-CoA and proceeds via HMG-CoA reductase (HMGCR), forming mevalonate and downstream intermediates (mevalonate-PP, isopentenyl-PP, and squalene). Squalene epoxidase (SQLE) converts squalene to 2,3(S)-oxidosqualene, leading to lanosterol and cholesterol synthesis. Fatty acid biosynthesis also originates from acetyl-CoA, with acetyl-CoA carboxylase (ACC) producing malonyl-CoA, followed by fatty acid synthase (FASN) generating palmitate and complex fatty acids. SREBP1/2 transcription factors regulate these pathways, with SREBP1 driving fatty acid synthesis and SREBP2 controlling cholesterol biosynthesis. The LDL receptor (LDLR) mediates cholesterol uptake, maintaining lipid homeostasis. Made using Biorender. ( B ) mRNA levels of HMGCR (HMG-CoA reductase), SQLE (Squalene Epoxidase), LDLR (Low density lipoprotein receptor), SREBP1 and SREBP2 (Sterol regulatory element-binding protein 1 or 2) relative to GAPDH on day 8 of differentiation after CD34 + isolation. A parametric Brown-Forsythe and Welch test was performed to test for differences between groups. p < 0.05 was considered statistically significant ( n = 3, N = 3). ( C ) Example waterfall plots of cells the same donor on day 8 of erythroid differentiation, labelled against Band3 and <t>CD49d,</t> grown in medium containing AB serum, Plasma, or Plasma supplemented with CRL. Corresponding percentage of each stage (I-Proerythoblast, II-Basophilic, III-Polychromatic, IV-Orthochromatic, and V-Reticulocyte) is indicated next to the corresponding gate. D Shows percentage of erythroid cell types present on day 8 for each condition studied, quantified as displayed in panel above ( n = 3). Error bars represent the standard deviation.
172yb Cx3cr1 2a9 1 Fluidigm 3172017b 173yb Granzyme Gb11 Fluidigm 3173006b B 174yb Cd49d 9f10 Fluidigm 3174018b, supplied by fluidigm, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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172yb cx3cr1 2a9 1 fluidigm 3172017b 173yb granzyme gb11 fluidigm 3173006b b 174yb cd49d 9f10 fluidigm 3174018b - by Bioz Stars, 2026-03
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cd49d 141pr cd49d 141pr false 9f10 hu dvs fluidigm 3141004b 257 cd3  (fluidigm)
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fluidigm cd49d 141pr cd49d 141pr false 9f10 hu dvs fluidigm 3141004b 257 cd3
qPCR shows cholesterol biosynthesis is significantly increased in plasma cultured cells. ( A ) Main steps in cholesterol and fatty acid biosynthesis. Cholesterol biosynthesis begins with acetyl-CoA and proceeds via HMG-CoA reductase (HMGCR), forming mevalonate and downstream intermediates (mevalonate-PP, isopentenyl-PP, and squalene). Squalene epoxidase (SQLE) converts squalene to 2,3(S)-oxidosqualene, leading to lanosterol and cholesterol synthesis. Fatty acid biosynthesis also originates from acetyl-CoA, with acetyl-CoA carboxylase (ACC) producing malonyl-CoA, followed by fatty acid synthase (FASN) generating palmitate and complex fatty acids. SREBP1/2 transcription factors regulate these pathways, with SREBP1 driving fatty acid synthesis and SREBP2 controlling cholesterol biosynthesis. The LDL receptor (LDLR) mediates cholesterol uptake, maintaining lipid homeostasis. Made using Biorender. ( B ) mRNA levels of HMGCR (HMG-CoA reductase), SQLE (Squalene Epoxidase), LDLR (Low density lipoprotein receptor), SREBP1 and SREBP2 (Sterol regulatory element-binding protein 1 or 2) relative to GAPDH on day 8 of differentiation after CD34 + isolation. A parametric Brown-Forsythe and Welch test was performed to test for differences between groups. p < 0.05 was considered statistically significant ( n = 3, N = 3). ( C ) Example waterfall plots of cells the same donor on day 8 of erythroid differentiation, labelled against Band3 and <t>CD49d,</t> grown in medium containing AB serum, Plasma, or Plasma supplemented with CRL. Corresponding percentage of each stage (I-Proerythoblast, II-Basophilic, III-Polychromatic, IV-Orthochromatic, and V-Reticulocyte) is indicated next to the corresponding gate. D Shows percentage of erythroid cell types present on day 8 for each condition studied, quantified as displayed in panel above ( n = 3). Error bars represent the standard deviation.
Cd49d 141pr Cd49d 141pr False 9f10 Hu Dvs Fluidigm 3141004b 257 Cd3, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd49d pe  (Miltenyi Biotec)
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Miltenyi Biotec cd49d pe
qPCR shows cholesterol biosynthesis is significantly increased in plasma cultured cells. ( A ) Main steps in cholesterol and fatty acid biosynthesis. Cholesterol biosynthesis begins with acetyl-CoA and proceeds via HMG-CoA reductase (HMGCR), forming mevalonate and downstream intermediates (mevalonate-PP, isopentenyl-PP, and squalene). Squalene epoxidase (SQLE) converts squalene to 2,3(S)-oxidosqualene, leading to lanosterol and cholesterol synthesis. Fatty acid biosynthesis also originates from acetyl-CoA, with acetyl-CoA carboxylase (ACC) producing malonyl-CoA, followed by fatty acid synthase (FASN) generating palmitate and complex fatty acids. SREBP1/2 transcription factors regulate these pathways, with SREBP1 driving fatty acid synthesis and SREBP2 controlling cholesterol biosynthesis. The LDL receptor (LDLR) mediates cholesterol uptake, maintaining lipid homeostasis. Made using Biorender. ( B ) mRNA levels of HMGCR (HMG-CoA reductase), SQLE (Squalene Epoxidase), LDLR (Low density lipoprotein receptor), SREBP1 and SREBP2 (Sterol regulatory element-binding protein 1 or 2) relative to GAPDH on day 8 of differentiation after CD34 + isolation. A parametric Brown-Forsythe and Welch test was performed to test for differences between groups. p < 0.05 was considered statistically significant ( n = 3, N = 3). ( C ) Example waterfall plots of cells the same donor on day 8 of erythroid differentiation, labelled against Band3 and <t>CD49d,</t> grown in medium containing AB serum, Plasma, or Plasma supplemented with CRL. Corresponding percentage of each stage (I-Proerythoblast, II-Basophilic, III-Polychromatic, IV-Orthochromatic, and V-Reticulocyte) is indicated next to the corresponding gate. D Shows percentage of erythroid cell types present on day 8 for each condition studied, quantified as displayed in panel above ( n = 3). Error bars represent the standard deviation.
Cd49d Pe, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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9f10  (fluidigm)
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fluidigm 9f10
qPCR shows cholesterol biosynthesis is significantly increased in plasma cultured cells. ( A ) Main steps in cholesterol and fatty acid biosynthesis. Cholesterol biosynthesis begins with acetyl-CoA and proceeds via HMG-CoA reductase (HMGCR), forming mevalonate and downstream intermediates (mevalonate-PP, isopentenyl-PP, and squalene). Squalene epoxidase (SQLE) converts squalene to 2,3(S)-oxidosqualene, leading to lanosterol and cholesterol synthesis. Fatty acid biosynthesis also originates from acetyl-CoA, with acetyl-CoA carboxylase (ACC) producing malonyl-CoA, followed by fatty acid synthase (FASN) generating palmitate and complex fatty acids. SREBP1/2 transcription factors regulate these pathways, with SREBP1 driving fatty acid synthesis and SREBP2 controlling cholesterol biosynthesis. The LDL receptor (LDLR) mediates cholesterol uptake, maintaining lipid homeostasis. Made using Biorender. ( B ) mRNA levels of HMGCR (HMG-CoA reductase), SQLE (Squalene Epoxidase), LDLR (Low density lipoprotein receptor), SREBP1 and SREBP2 (Sterol regulatory element-binding protein 1 or 2) relative to GAPDH on day 8 of differentiation after CD34 + isolation. A parametric Brown-Forsythe and Welch test was performed to test for differences between groups. p < 0.05 was considered statistically significant ( n = 3, N = 3). ( C ) Example waterfall plots of cells the same donor on day 8 of erythroid differentiation, labelled against Band3 and <t>CD49d,</t> grown in medium containing AB serum, Plasma, or Plasma supplemented with CRL. Corresponding percentage of each stage (I-Proerythoblast, II-Basophilic, III-Polychromatic, IV-Orthochromatic, and V-Reticulocyte) is indicated next to the corresponding gate. D Shows percentage of erythroid cell types present on day 8 for each condition studied, quantified as displayed in panel above ( n = 3). Error bars represent the standard deviation.
9f10, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3174018c  (fluidigm)
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fluidigm 3174018c
qPCR shows cholesterol biosynthesis is significantly increased in plasma cultured cells. ( A ) Main steps in cholesterol and fatty acid biosynthesis. Cholesterol biosynthesis begins with acetyl-CoA and proceeds via HMG-CoA reductase (HMGCR), forming mevalonate and downstream intermediates (mevalonate-PP, isopentenyl-PP, and squalene). Squalene epoxidase (SQLE) converts squalene to 2,3(S)-oxidosqualene, leading to lanosterol and cholesterol synthesis. Fatty acid biosynthesis also originates from acetyl-CoA, with acetyl-CoA carboxylase (ACC) producing malonyl-CoA, followed by fatty acid synthase (FASN) generating palmitate and complex fatty acids. SREBP1/2 transcription factors regulate these pathways, with SREBP1 driving fatty acid synthesis and SREBP2 controlling cholesterol biosynthesis. The LDL receptor (LDLR) mediates cholesterol uptake, maintaining lipid homeostasis. Made using Biorender. ( B ) mRNA levels of HMGCR (HMG-CoA reductase), SQLE (Squalene Epoxidase), LDLR (Low density lipoprotein receptor), SREBP1 and SREBP2 (Sterol regulatory element-binding protein 1 or 2) relative to GAPDH on day 8 of differentiation after CD34 + isolation. A parametric Brown-Forsythe and Welch test was performed to test for differences between groups. p < 0.05 was considered statistically significant ( n = 3, N = 3). ( C ) Example waterfall plots of cells the same donor on day 8 of erythroid differentiation, labelled against Band3 and <t>CD49d,</t> grown in medium containing AB serum, Plasma, or Plasma supplemented with CRL. Corresponding percentage of each stage (I-Proerythoblast, II-Basophilic, III-Polychromatic, IV-Orthochromatic, and V-Reticulocyte) is indicated next to the corresponding gate. D Shows percentage of erythroid cell types present on day 8 for each condition studied, quantified as displayed in panel above ( n = 3). Error bars represent the standard deviation.
3174018c, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd49d  (fluidigm)
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fluidigm cd49d
qPCR shows cholesterol biosynthesis is significantly increased in plasma cultured cells. ( A ) Main steps in cholesterol and fatty acid biosynthesis. Cholesterol biosynthesis begins with acetyl-CoA and proceeds via HMG-CoA reductase (HMGCR), forming mevalonate and downstream intermediates (mevalonate-PP, isopentenyl-PP, and squalene). Squalene epoxidase (SQLE) converts squalene to 2,3(S)-oxidosqualene, leading to lanosterol and cholesterol synthesis. Fatty acid biosynthesis also originates from acetyl-CoA, with acetyl-CoA carboxylase (ACC) producing malonyl-CoA, followed by fatty acid synthase (FASN) generating palmitate and complex fatty acids. SREBP1/2 transcription factors regulate these pathways, with SREBP1 driving fatty acid synthesis and SREBP2 controlling cholesterol biosynthesis. The LDL receptor (LDLR) mediates cholesterol uptake, maintaining lipid homeostasis. Made using Biorender. ( B ) mRNA levels of HMGCR (HMG-CoA reductase), SQLE (Squalene Epoxidase), LDLR (Low density lipoprotein receptor), SREBP1 and SREBP2 (Sterol regulatory element-binding protein 1 or 2) relative to GAPDH on day 8 of differentiation after CD34 + isolation. A parametric Brown-Forsythe and Welch test was performed to test for differences between groups. p < 0.05 was considered statistically significant ( n = 3, N = 3). ( C ) Example waterfall plots of cells the same donor on day 8 of erythroid differentiation, labelled against Band3 and <t>CD49d,</t> grown in medium containing AB serum, Plasma, or Plasma supplemented with CRL. Corresponding percentage of each stage (I-Proerythoblast, II-Basophilic, III-Polychromatic, IV-Orthochromatic, and V-Reticulocyte) is indicated next to the corresponding gate. D Shows percentage of erythroid cell types present on day 8 for each condition studied, quantified as displayed in panel above ( n = 3). Error bars represent the standard deviation.
Cd49d, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd49d/product/fluidigm
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reafinitytm  (Miltenyi Biotec)
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Miltenyi Biotec reafinitytm

Reafinitytm, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd49d  (Miltenyi Biotec)
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Miltenyi Biotec cd49d

Cd49d, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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qPCR shows cholesterol biosynthesis is significantly increased in plasma cultured cells. ( A ) Main steps in cholesterol and fatty acid biosynthesis. Cholesterol biosynthesis begins with acetyl-CoA and proceeds via HMG-CoA reductase (HMGCR), forming mevalonate and downstream intermediates (mevalonate-PP, isopentenyl-PP, and squalene). Squalene epoxidase (SQLE) converts squalene to 2,3(S)-oxidosqualene, leading to lanosterol and cholesterol synthesis. Fatty acid biosynthesis also originates from acetyl-CoA, with acetyl-CoA carboxylase (ACC) producing malonyl-CoA, followed by fatty acid synthase (FASN) generating palmitate and complex fatty acids. SREBP1/2 transcription factors regulate these pathways, with SREBP1 driving fatty acid synthesis and SREBP2 controlling cholesterol biosynthesis. The LDL receptor (LDLR) mediates cholesterol uptake, maintaining lipid homeostasis. Made using Biorender. ( B ) mRNA levels of HMGCR (HMG-CoA reductase), SQLE (Squalene Epoxidase), LDLR (Low density lipoprotein receptor), SREBP1 and SREBP2 (Sterol regulatory element-binding protein 1 or 2) relative to GAPDH on day 8 of differentiation after CD34 + isolation. A parametric Brown-Forsythe and Welch test was performed to test for differences between groups. p < 0.05 was considered statistically significant ( n = 3, N = 3). ( C ) Example waterfall plots of cells the same donor on day 8 of erythroid differentiation, labelled against Band3 and CD49d, grown in medium containing AB serum, Plasma, or Plasma supplemented with CRL. Corresponding percentage of each stage (I-Proerythoblast, II-Basophilic, III-Polychromatic, IV-Orthochromatic, and V-Reticulocyte) is indicated next to the corresponding gate. D Shows percentage of erythroid cell types present on day 8 for each condition studied, quantified as displayed in panel above ( n = 3). Error bars represent the standard deviation.

Journal: Scientific Reports

Article Title: Choice of lipid supplementation for in vitro erythroid cell culture impacts reticulocyte yield and characteristics

doi: 10.1038/s41598-026-37229-z

Figure Lengend Snippet: qPCR shows cholesterol biosynthesis is significantly increased in plasma cultured cells. ( A ) Main steps in cholesterol and fatty acid biosynthesis. Cholesterol biosynthesis begins with acetyl-CoA and proceeds via HMG-CoA reductase (HMGCR), forming mevalonate and downstream intermediates (mevalonate-PP, isopentenyl-PP, and squalene). Squalene epoxidase (SQLE) converts squalene to 2,3(S)-oxidosqualene, leading to lanosterol and cholesterol synthesis. Fatty acid biosynthesis also originates from acetyl-CoA, with acetyl-CoA carboxylase (ACC) producing malonyl-CoA, followed by fatty acid synthase (FASN) generating palmitate and complex fatty acids. SREBP1/2 transcription factors regulate these pathways, with SREBP1 driving fatty acid synthesis and SREBP2 controlling cholesterol biosynthesis. The LDL receptor (LDLR) mediates cholesterol uptake, maintaining lipid homeostasis. Made using Biorender. ( B ) mRNA levels of HMGCR (HMG-CoA reductase), SQLE (Squalene Epoxidase), LDLR (Low density lipoprotein receptor), SREBP1 and SREBP2 (Sterol regulatory element-binding protein 1 or 2) relative to GAPDH on day 8 of differentiation after CD34 + isolation. A parametric Brown-Forsythe and Welch test was performed to test for differences between groups. p < 0.05 was considered statistically significant ( n = 3, N = 3). ( C ) Example waterfall plots of cells the same donor on day 8 of erythroid differentiation, labelled against Band3 and CD49d, grown in medium containing AB serum, Plasma, or Plasma supplemented with CRL. Corresponding percentage of each stage (I-Proerythoblast, II-Basophilic, III-Polychromatic, IV-Orthochromatic, and V-Reticulocyte) is indicated next to the corresponding gate. D Shows percentage of erythroid cell types present on day 8 for each condition studied, quantified as displayed in panel above ( n = 3). Error bars represent the standard deviation.

Article Snippet: CD49d was detected using a FITC-conjugated anti-human CD49d antibody (clone MZ18-24A9), a mouse IgG2b from Miltenyi Biotech.

Techniques: Clinical Proteomics, Cell Culture, Binding Assay, Isolation, Standard Deviation

Journal: Cell Reports Methods

Article Title: Dynamic stimulation promotes functional tissue-like organization of a 3D human lymphoid microenvironment model in vitro

doi: 10.1016/j.crmeth.2025.101105

Figure Lengend Snippet:

Article Snippet: CD49d Antibody, anti-human, REAfinityTM , Miltenyi , CAT#130-127-192 RRID: AB_2905060.

Techniques: Knock-Out, Recombinant, Virus, Plasmid Preparation, Binding Assay, Alamar Blue Assay, Imaging, cDNA Synthesis, Staining, Software, In Vitro